Summary information and primary citation
- PDB-id
-
2h1m;
DSSR-derived features in text and
JSON formats
- Class
- RNA
- Method
- X-ray (2.9 Å)
- Summary
- Synthesis, oxidation behavior, crystallization and
structure of 2'-methylseleno guanosine containing rnas
- Reference
-
Moroder H, Kreutz C, Lang K, Serganov A, Micura R (2006):
"Synthesis,
Oxidation Behavior, Crystallization and Structure of
2'-Methylseleno Guanosine Containing RNAs."
J.Am.Chem.Soc., 128, 9909-9918.
doi: 10.1021/ja0621400.
- Abstract
- We have recently introduced a basic concept for the
combined chemical and enzymatic preparation of
site-specifically modified 2'-methylseleno RNAs which
represent useful derivatives for phasing of X-ray
crystallographic data during their three-dimensional
structure determination. Here, we introduce the first
synthesis of an appropriate guanosine phosphoramidite,
which complements the thus far established set of
2'-methylseleno-modified uridine, cytidine, and adenosine
building blocks for solid-phase synthesis. The novel
building block was readily incorporated into RNA.
Importantly, it was the 2'-methylseleno-guanosine-labeled
RNA that allowed us to reveal the reversible
oxidation/reduction behavior of the Se moiety and thus it
represents a valuable contribution to the understanding of
the action of threo-1,4-dimercapto-2,3-butanediol (DTT)
required during solid-phase synthesis, deprotection, and
crystallization of selenium-containing RNA. In addition, we
investigated 2'-methylseleno RNA with respect to
crystallization properties. Our studies revealed that the
Se modification significantly increases the range of
conditions leading to crystal growth. Moreover, we
determined the crystal structures of model RNA helices and
showed that the Se modification can affect crystal packing
interactions, thus potentially expanding the possibilities
for obtaining the best crystal form.
