DSSR-PyMOL cartoon-block schematics: PDB entry 5o7t

PDB-id

5o7t; DSSR-derived features in text and JSON formats

Class

transferase

Method

X-ray (1.8 Å)

Summary

Crystal structure of klentaq mutant m747k in a closed ternary complex with a dg:dctp base pair

Reference

Betz K, Nilforoushan A, Wyss LA, Diederichs K, Sturla SJ, Marx A (2017): "Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase." Chem. Commun. (Camb.), 53, 12704-12707. doi: 10.1039/c7cc07173f.

Abstract

The possibility to sequence cytotoxic O₆-alkylG DNA adducts would greatly benefit research. Recently we reported a benzimidazole-derived nucleotide that is selectively incorporated opposite the damaged site by a mutated DNA polymerase. Here we provide the structural basis for this reaction which may spur future developments in DNA damage sequencing.