Summary information and primary citation
- PDB-id
-
7nas;
DSSR-derived features in text and
JSON formats
- Class
- ribosome
- Method
- cryo-EM (3.31 Å)
- Summary
- Bacterial 30s ribosomal subunit assembly complex state
a (multibody refinement for body domain of 30s
ribosome)
- Reference
-
Schedlbauer A, Iturrioz I, Ochoa-Lizarralde B, Diercks T,
Lopez-Alonso JP, Lavin JL, Kaminishi T, Capuni R, Dhimole
N, de Astigarraga E, Gil-Carton D, Fucini P, Connell SR
(2021): "A
conserved rRNA switch is central to decoding site
maturation on the small ribosomal subunit." Sci
Adv, 7. doi: 10.1126/sciadv.abf7547.
- Abstract
- While a structural description of the molecular
mechanisms guiding ribosome assembly in eukaryotic systems
is emerging, bacteria use an unrelated core set of assembly
factors for which high-resolution structural information is
still missing. To address this, we used single-particle
cryo-electron microscopy to visualize the effects of
bacterial ribosome assembly factors RimP, RbfA, RsmA, and
RsgA on the conformational landscape of the
30<i>S</i> ribosomal subunit and obtained eight
snapshots representing late steps in the folding of the
decoding center. Analysis of these structures identifies a
conserved secondary structure switch in the
16<i>S</i> ribosomal RNA central to decoding
site maturation and suggests both a sequential order of
action and molecular mechanisms for the assembly factors in
coordinating and controlling this switch. Structural and
mechanistic parallels between bacterial and eukaryotic
systems indicate common folding features inherent to all
ribosomes.
