Summary information and primary citation
- PDB-id
-
9g7c;
DSSR-derived features in text and
JSON formats
- Class
- RNA
- Method
- cryo-EM (3.27 Å)
- Summary
- cryo-EM structure of raia ncrna from clostridium
reveals a new RNA 3d fold
- Reference
-
Badepally NG, de Moura TR, Purta E, Baulin EF, Bujnicki
JM (2024): "Cryo-EM
Structure of raiA ncRNA From Clostridium Reveals a New
RNA 3D Fold." J.Mol.Biol.,
436, 168833. doi: 10.1016/j.jmb.2024.168833.
- Abstract
- Advancements in genome-wide sequence analysis have led
to the discovery of numerous novel bacterial non-coding
RNAs (ncRNAs). These ncRNAs have been categorized into
various RNA families and classes based on their size,
structure, function, and evolutionary relationships. One
such ncRNA family, raiA, is notably abundant in the
bacterial phyla Firmicutes and Actinobacteria and is
remarkably well-conserved across many Gram-positive
bacteria. In this study, we integrated cryo-electron
microscopy single-particle analysis with computational
modeling and biochemical techniques to elucidate the
structural characteristics of raiA from Clostridium sp. CAG
138. Our findings reveal the globular 3D fold of raiA,
providing valuable structural insights. This analysis paves
the way for future investigations into the functional
properties of raiA, potentially uncovering new regulatory
mechanisms in bacterial ncRNAs.
